Research Articles | Challenge Journal of Perioperative Medicine

The effects of lycopene in lung injury associated with cecum ligation and perforation-induced sepsis in rats: An investigative animal experiment study

Sumeyra Zeren, Ozgur Ozmen, Zekai Halici, Sare Sipal, Betul Gundogdu, Kamber Kasali, Nazim Dogan, Husnu Kursad


DOI: https://doi.org/10.20528/cjpm.2025.02.001
View Counter: Abstract | 443 times | ‒ Full Article | 166 times |

Full Text:

PDF

Abstract


Background: The primary aim of this study is to evaluate the effect of lycopene on serum interleukin-1ß (IL-1ß) and interleukin-6 (IL-6) levels in rats subjected to sepsis induced by the cecal ligation and perforation (CLP) method, as well as to assess the impact of lycopene on inflammation in the lungs, which is the first organ affected by sepsis.

Methods: Twenty-four male rats were divided into four groups - control (healthy group), sepsis (CLP group), sepsis + lycopene 100 mg/kg (L100 group), and sepsis + lycopene 200 mg/kg (L200 group). Lycopene was administered by gastric lavage at doses of 100 mg/kg to the L100 group and 200 mg/kg to the L200 group. Intracardiac blood samples were collected 18 h after CLP for serum IL-1β and IL-6 level analysis. Lung tissue specimens were also collected for histopathological examination.

Results: IL-1β levels decreased significantly in the L100 and L200 groups compared to the CLP group (p<0.001). Both doses of lycopene statistically significantly reduced serum IL-6 levels in the L100 and L200 groups compared to the CLP group. Serum IL-6 levels also decreased significantly in the L200 group compared to the L100 group (p<0.001).The degree of inflammation, vascular congestion and edema decreased significantly in the L200 group compared to the CLP group (p<0.001).

Conclusion: Use of lycopene in rats with CLP-induced sepsis reduced serum IL-1β and IL-6 levels and inflammation in lung tissue at histopathological examination. Lycopene can be more effective at a dosage of 200 mg/kg.


Keywords


sepsis; lycopene; interleukin-1β; interleukin-6; lung damage

References


Rhodes A, Ewans LE, Alhazzani W, et al. Surviving Sepsis Campaign: International guidelines for management of sepsis and septic shock: 2016. Intensive Care Med. 2017;43:304–377.

Bone RC, Grodzin CJ, Balk RA. Sepsis: A new hypothesis for pathogenesis of the disease process. Chest. 1997;112:235–243.

Zilbelberg MD, Shorr AF, Micek ST, et al. Multi-drug resistance, inappropriate initial antibiotic therapy and mortality in Gram-negative severe sepsis and septic shock: a retrospective cohort study. Crit Care. 2014;18:596.

Bernard GR, Vincent JL, Laterre PF, et al. Efficacy and safety of recombinant human activated protein C for severe sepsis. N Engl J Med. 2001;344:699–709.

Pool R, Gomez H, Kellum JA. Mechanisms of organ dysfunction in sepsis. Crit Care Clin. 2018;34:63–80.

Karakike E, Giamarellos-Bourboulis EJ. Macrophage activation-like syndrome: a distinct entity leading to early death in sepsis. Front Immunol. 2019;10:55.

Bozza FA, Salluh JI, Japiassu AM, et al. Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis. Crit Care. 2007;11:R49.

Bhuvaneswari V, Nagini S. Lycopene: a review of its potential as an anticancer agent. Curr Med Chem Anticancer Agents. 2005;5:627–635.

Cadirci E, Ugan RA, Dincer B, et al. Urotensin receptors as a new target for CLP induced septic lung injury in mice. Naunyn Schmiedebergs Arch Pharmacol. 2019;392:135–145.

Herrán-Monge R, Muriel-Bombín A, García-García MM, et al. Grecia network epidemiology and changes in mortality of sepsis after the implementation of Surviving Sepsis Campaign guidelines. J Intensive Care Med. 2019;34:740–750.

Yébenes JC, Ruiz-Rodriguez JC, Ferrer R, et al. Epidemiology of sepsis in Catalonia: analysis of incidence and outcomes in a European setting. Ann Intensive Care. 2017;7:19.

Poli-de-Figueiredo LF, Garrido AG, Nakagawa N, et al. Experimental models of sepsis and their clinical relevance. Shock. 2008;30:53–59.

Ding LA, Li JS, Li YS, et al. Intestinal barrier damage caused by trauma and lipopolysaccharide. World J Gastroenterol. 2004;10(16):2373–2378.

Yang S, Koo DJ, Chaudry JH, et al. The important role of the gut in initiating the hyperdynamic response during early sepsis. J Surg Res. 2000;89:31–37.

Zhang H, Slutsky AS, Vincent JL. Oxygen free radicals in ARDS, septic shock and organ dysfunction. Intensive Care Med. 2000;26:474–476.

Qin X, Jiang X, et al. Micheliolide inhibits LPS-induced inflammatory response and protects mice from LPS challenge. Sci Rep. 2016;6:23240.

Huang H, Tu L. Expression of S100 family proteins in neonatal rats with sepsis and its significance. Int J Clin Exp Pathol. 2015;8(2):1631–1639.

Pruthi RS, Derksen E, Gaston K. Cyclooxygenase-2 as a potential target in the prevention and treatment of genitourinary tumors: a review. J Urol. 2003;169:2352–2359.

Huang CS, Fan YE, Lin CY, et al. Lycopene inhibits matrix metalloproteinase-9 expression and down-regulates the binding activity of nuclear factor-kappa B and stimulatory protein-1. J Nutr Biochem. 2007;18:449.

Chung KF. Cytokines as targets in chronic obstructive pulmonary disease. Curr Drug Targets. 2006;7:675–681.

Simone RE, Russo M, Catalano A, et al. Lycopene inhibits NF-kB-mediated IL-8 expression and changes redox and PPARγ signalling in cigarette smoke–stimulated macrophages. PLoS One. 2011;6:e19652.

Giovannucci E. Tomatoes, tomato-based products, lycopene, and cancer: review of the epidemiologic literature. J Natl Cancer Inst. 1999;91(4):317–331.

Kuçuk O, Sarkar H, Sakr W, et al. Phase II randomized clinical trial of lycopene supplementation before radical prostatectomy. Cancer Epidemiol Biomarkers Prev. 2001;10:861–868.

Jamshidzadeh A, Baghban M, Azarpira N, et al. Effects of tomato extract on oxidative stress induced toxicity in different organs of rats. Food Chem Toxicol. 2008;46:3612–3615.

Petyaev IM. Lycopene deficiency in ageing and cardiovascular disease. Oxid Med Cell Longev. 2016;2016:3218605.

Osinska AN, Begier-Krasinska B, Rzymski P, et al. The influence of adding tomato extract and acetylsalicylic acid to hypotensive therapy on the daily blood pressure profiles of patients with arterial hypertension and high cardiovascular risk. Kardiochir Torakochir Pol. 2017;14:245–252.

Li W, Wang G, Lu X, et al. Lycopene ameliorates renal function in rats with streptozotocin-induced diabetes. Int J Clin Exp Pathol. 2014;7:5008–5015.

Dieleman LA, Goerres MS, Arends A, et al. Lactobacillus GG prevents recurrence of colitis in HLA-B27 transgenic rats after antibiotic treatment. Gut. 2003;52:370–376.

Reifen R, Nissenkorn A, Matas Z, et al. 5-ASA and lycopene decrease the oxidative stress and inflammation induced by iron in rats with colitis. J Gastroenterol. 2004;39:514–519.

Turkoglu S, Muz MH, Özercan R, et al. Effects of lycopene on the model of oleic acid induced acute lung injury. Tuberk Toraks. 2012;60:101–107.

Hua S, Liu X, Lv S, et al. Protective effects of Cucurbitacin B on acute lung injury induced by sepsis in rats. Med Sci Monit. 2017;23:1355–1362.

Demir M, Taylan M, Kaya H, et al. Histopathological and biochemical effects of Ecballium elaterium on sepsis-induced lung injury. J Investig Surg. 2016;29:302–308.


Refbacks

  • There are currently no refbacks.